Amino acid analysis liquid chromatography (detecting vitamin and amino acid content)It is a high-performance liquid chromatography instrument developed and designed by the company based on customer needs, utilizing advanced foreign technology, and manufactured domestically. This instrument is a high-performance gradient elution analysis system that can perform binary high-pressure gradient elution and can also be expanded into a preparation system, including a high-pressure infusion pump PT501, a built-in fixed mixer pump PT501, a UV-501 detector, a 7725i injection valve, a chromatographic column, etc. The instrument adopts an imported high-pressure mixer connected to two high-pressure constant current pumps, and uses RS232 communication to achieve gradient analysis, making it easy for users to freely choose and match. The high-pressure mixer has a complete range of types, including analytical, high flow rate, low flow rate, and other series, covering the common needs of users. We also offer a series of stirred high-pressure mixers to meet the special needs of users. The high-pressure mixer is designed as a separate component for users to choose from, with quick replacement and easy operation. The mixing equipment is mainly composed of stainless steel, bio inert PEEK, etc., and has a wide range of applications. The STI501 gradient high-performance liquid chromatograph has excellent characteristics such as stable quality, superior performance, and extremely high cost-effectiveness, and can be widely used in many analytical fields such as research and development, pharmaceutical inspection, food testing, chemical analysis, environmental monitoring, etc.
Amino acid analysis liquid chromatographySystem configuration (measuring amino acids and vitamins)
Product Name |
Specification and model |
Company |
quantity |
amount of money |
Place of Origin |
High pressure constant flow pump |
10T |
platform |
2 |
55000 |
Precision measurement |
UV detector |
10T |
platform |
1 |
Precision measurement |
|
Manual injection valve |
8725i |
only |
1 |
Precision measurement |
|
system controller |
Including workstation VI2010 (dedicated for liquid phase) |
set |
1 |
Precision measurement |
|
mixer |
special-purpose |
only |
1 |
Precision measurement |
|
Backup damper |
special-purpose |
only |
1 |
Precision measurement |
|
Spiral tube |
SS stainless steel 1/16 × 0.04 3m |
set |
1 |
U.S.A |
|
Liquid chromatography column |
C18 4.6×250mm,5um |
branch |
1 |
VERTEX |
|
Microsampler |
50ul/100ul |
branch |
1 |
Shanghai |
|
Reverse pressure valve |
|
only |
1 |
Precision measurement |
|
Injection bracket |
For injection valve |
only |
1 |
Precision measurement |
|
Column temperature box |
DT230A |
platform |
1 |
1250 |
Dalian |
Vacuum pump |
AP-01P (with pressure gauge) |
platform |
1 |
1100 |
Tianjin |
Solvent filter |
1000ml |
platform |
1 |
200 |
Jiangsu |
Ultrasonic degassing machine |
SZ-80(2L) |
platform |
1 |
500 |
Tianjin |
Water based microporous filter |
Φ 13 × 0.45 (100 pieces/pack) |
package |
1 |
40 |
Tianjin |
Organic microporous filter |
Φ 13 × 0.45 (100 pieces/pack) |
package |
1 |
40 |
Tianjin |
Water based microporous filter membrane |
Φ 50 × 0.45 (50 pieces/box) |
box |
1 |
25 |
Tianjin |
Organic microporous membrane |
Φ 50 × 0.45 (50 pieces/box) |
box |
1 |
35 |
Tianjin |
Amino acid analysis special package |
|
set |
1 |
3410 |
Shanghai |
Note: 1. This configuration can also measure vitamins.
2. Customers are required to prepare sample processing equipment such as scales that will be shared in the plan.
3. The analysis package contains dedicated chromatography columns, amino acid standards, derivative reagents, diluents, and other reagents to be provided by the customer.
(The configuration may vary slightly according to customer needs)
characteristic
1. Characteristics of high-pressure constant current infusion pump
The pump head has a self flushing function, allowing for infusion without the need for manual emptying;
The "self-priming one-way valve" technology that has won the design award can extend its service life;
Complete pump types: analytical, semi prepared, and prepared;
The gradient elution internal software can be self controlled;
Programmable solvents and compression factors that can be automatically compensated;
Various components are easy to maintain and repair;
Complete pre cleaning function, facilitating quick solvent replacement;
By controlling the closure of external contacts, it is easy to change the purpose of the pump;
The control method is flexible, and the pump parameters can be controlled by both the pump panel and the system controller under different requirements, making it convenient and flexible.
2. Characteristics of UV detector
Power on self-test, internal safety alarm and automatic fault diagnosis function of the instrument;
The optical path system adopts precise positioning structure and unique heat dissipation technology, efficient optical system and digital filtering;
Light source: optional deuterium lamp and tungsten lamp, deuterium lamp warranty life of 1000Hrs;
Original imported light source, no need to adjust the optical axis when replacing the light source, easy maintenance;
Real time display of sample and reference cell energy, facilitating the determination of light source and flow cell status;
Online monitoring and display of the running time of the light source, making it convenient for users to determine the lifespan of the light source;
Zeroing function: The absorbance can be zeroed by the panel or automatically zeroed by the program software;
Wavelength adjustment function: It can be controlled by the keyboard panel or set by program software. The integrated photoelectric detection element can select or change the wavelength without any mechanical adjustment.
Technical indicators
PT501 high-pressure constant current infusion pump | |
Infusion method |
Micro volume series double plunger |
Flow range |
(0.001~10.000) mL/min, with a small increment of 0.001 mL/min (analytical type). |
Flow accuracy |
0.1% |
Traffic accuracy |
<±1 % (2μl/min) |
Maximum conveying pressure |
6000PSi (approximately 42MPa) |
Solvent compressibility |
Automatic compensation |
Security |
Equipped with power on self-test, high and low voltage alarm and protection functions. |
size |
W260×H170×D360mm |
weight |
6.3kg |
UV variable wavelength detector | |
light source |
Deuterium lamp (tungsten lamp optional) |
Wavelength Range |
190-700nm (step up by 1nm), can be set by keyboard or RS-232. |
Wavelength accuracy |
<±1nm |
Wavelength reproducibility |
<±0.5nm |
bandwidth |
Less than 5nm |
noise |
<± 2.5 × 10-5AU (under specific conditions) |
drift |
<± 2.5 × 10-4AU (under specific conditions) |
Flow pool volume |
10ul |
Absorption range |
0.01AU/V to 10AU/V (ten levels in total) |
size |
W260×H170×D360mm |
weight |
6.0kg |
Software Introduction
The gradient high-performance liquid chromatograph adopts the Zhejiang University N2000 chromatographic data workstation.The N2000 chromatographic data workstation, developed and promoted by Saizhi Technology (formerly Zhejiang University Zhida Information Engineering Co., Ltd.) for many years, has become the largest market share and most cost-effective chromatographic data workstation in China. The software interface is simple, clear, simple and practical, and is highly favored by users.
Attachment: Technical specifications of N2000 chromatography workstation
Software:
1. Automatically identify solvent peaks, tailing peaks, sawtooth peaks, and front and back shoulder peaks.
2. Automatically adjust parameters (peak width, slope) during the analysis process.
3. Baseline automatic tracking, automatic classification of chromatographic peak types; Peak rise and fall points.
4. Powerful manual integration function, peak addition and subtraction, peak baseline adjustment, and cutting method adjustment.
5. Integral sensitivity of 1 microvolt • second, minimum resolution of 0.1 microvolt, minimum peak width of 0.1 second.
6. The data results can be shared with software such as Microsoft Excel and Word; The data spectrum can be shared with image software such as Photoshop and CorelDRAW.
7. Data reanalysis function, archiving.
8. Quantitative calculation methods: normalization method, calibration normalization method, internal standard method, grouping method, external standard method, index method.
Hardware:
1. Dual channel, external type.
2. Output -5mv-1.7v.
3. The input impedance is greater than 10 megaohms.
4. Dynamic range 107, linearity ± 0.1%.
5. The sampling frequency is 10, 25, and 50 times per second.
6. RS232 communication method, key remote control, keyboard shortcut keys or mouse menu can also be used.